Melting Temperature of Nucleic Acid Sequences

The melting temperature of nucleic acid sequences can be calculated in three method, the Wallace rule (Thein & Wallace (1986) ), empirical formulas based on G and C content (Marmur J. (1962) , Schildkraut C. (2010) , Wetmur J G (1991) <10.3109/10409239109114069>, Untergasser,A. (2012) , von Ahsen N (2001) ) and nearest neighbor thermodynamics (Breslauer K J (1986) , Sugimoto N (1996) , Allawi H (1998) , SantaLucia J (2004) , Freier S (1986) , Xia T (1998) , Chen JL (2012) , Bommarito S (2000) , Turner D H (2010) , Sugimoto N (1995) , Allawi H T (1997) , Santalucia N (2005) ), and it can also be corrected with salt ions and chemical compound (SantaLucia J (1996) , SantaLucia J(1998) , Owczarzy R (2004) , Owczarzy R (2008) ).