High Throughput Sequencing of Stable Isotope Probing Data Analysis

Functions for analyzing high throughput sequencing stable isotope probing (HTS-SIP) data. Analyses include high resolution stable isotope probing (HR-SIP), multi-window high resolution stable isotope probing (MW-HR-SIP), and quantitative stable isotope probing (q-SIP).

HTSSIP

HTSSIP is an R package for analyzing high throughput sequence data from nucleotide stable isotope probing (DNA- & RNA-SIP) experiments.

Available analyzes

• Identifying community-level isotope incorporatation

  • Ordinations of gradient fraction communities
  • Beta diversity of overlapping gradient fractions

• Identifying isotope incorporators

  • High resolution stable isotope probing (HR-SIP)
  • Multiple window high resolution stable isotope probing (MW-HR-SIP)
  • Quantitative stable isotope probing (q-SIP)

Documentation

All documentation can be found on CRAN.

A good place to start is the HTSSIP introduction vignette.

The manuscript describing HTSSIP is:

Youngblut ND, Barnett SE, Buckley DH (2018) HTSSIP: An R package for analysis of high throughput sequencing data from nucleic acid stable isotope probing (SIP) experiments. PLoS ONE 13(1): e0189616. https://doi.org/10.1371/journal.pone.0189616

References

See References in the HTSSIP introduction vignette.

Installation

To get the current released version from CRAN:

install.packages("HTSSIP") 

To get the current development version from github:

# install.packages("devtools")
devtools::install_github("buckleylab/HTSSIP")