Guide Sequence Design for CRISPR/Cas9

Designs guide sequences for CRISPR/Cas9 genome editing and provides information on sequence features pertinent to guide efficiency. Sequence features include annotated off-target predictions in a user-selected genome and a predicted efficiency score based on the model described in Doench et al. (2016) . Users are able to import additional genomes and genome annotation files to use when searching and annotating off-target hits. All guide sequences and off-target data can be generated through the 'R' console with sgRNA_Design() or through 'crispRdesignR's' user interface with crispRdesignRUI(). CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the associated protein Cas9 refer to a technique used in genome editing.


Reference manual

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1.1.6 by Dylan Beeber, a year ago


Browse source code at

Authors: Dylan Beeber [aut, cre] , Frederic Chain [aut]

Documentation:   PDF Manual  

GPL-3 license

Imports Biostrings, gbm, GenomicRanges, BiocGenerics, IRanges, GenomeInfoDb, S4Vectors, rtracklayer, stringr, vtreat, shiny, DT

Suggests BSgenome.Scerevisiae.UCSC.sacCer2

See at CRAN