Guide Sequence Design for CRISPR/Cas9
Designs guide sequences for CRISPR/Cas9 genome editing and
provides information on sequence features pertinent to guide
efficiency. Sequence features include annotated off-target
predictions in a user-selected genome and a predicted efficiency
score based on the model described in Doench et al. (2016)
. Users are able to import additional genomes
and genome annotation files to use when searching and annotating
off-target hits. All guide sequences and off-target data can be
generated through the 'R' console with sgRNA_Design() or through
'crispRdesignR's' user interface with crispRdesignRUI(). CRISPR
(Clustered Regularly Interspaced Short Palindromic Repeats) and the
associated protein Cas9 refer to a technique used in genome editing.