Analysis of Multiplex Cytometric Bead Assays

Reproducible and automated analysis of multiplex bead assays such as CBA (Morgan et al. 2004; ), LEGENDplex (Yu et al. 2015; ), and MACSPlex (Miltenyi Biotec 2014; Application note: Data acquisition and analysis without the MACSQuant analyzer; <>). The package provides functions for streamlined reading of fcs files, and identification of bead clusters and analyte expression. The package eases the calculation of standard curves and the subsequent calculation of the analyte concentration.

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Reproducible and easy analysis and evaluation of LEGENDplex, CBA, and MACSPlex experiments.

The companies behind the above beadplex systems, provide their own software for the analysis of the generated data. However, the strong dependence on manual interaction make the respective solutions labour insensitive and above all not always reproducible.


# The development version from GitHub:
# install.packages("devtools")
# Or with vignettes built
# devtools::install_github("ustervbo/beadplexr", build_vignettes = TRUE)

Getting started


The package comes with a comprehensive example in the vignette 'Analysis of LEGENDplex data with beadplexr':



beadplexr version 0.1.0

Initial release - clip the flag!

Reference manual

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0.2.0 by Ulrik Stervbo, 2 months ago

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Browse source code at

Authors: Ulrik Stervbo [aut, cre]

Documentation:   PDF Manual  

MIT + file LICENSE license

Imports cluster, dplyr, drc, flowCore, fpc, ggplot2, lazyeval, magrittr, mclust, purrr, raster, tibble, tidyr, yaml

Suggests gridExtra, hexbin, igraph, knitr, rmarkdown, stringr, testthat

See at CRAN