Analysis of Multiplex Cytometric Bead Assays

Reproducible and automated analysis of multiplex bead assays such as CBA (Morgan et al. 2004; ), LEGENDplex (Yu et al. 2015; ), and MACSPlex (Miltenyi Biotec 2014; Application note: Data acquisition and analysis without the MACSQuant analyzer; < https://www.miltenyibiotec.com/upload/assets/IM0021608.PDF>). The package provides functions for streamlined reading of fcs files, and identification of bead clusters and analyte expression. The package eases the calculation of standard curves and the subsequent calculation of the analyte concentration.


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Reproducible and easy analysis and evaluation of LEGENDplex, CBA, and MACSPlex experiments.

The companies behind the above beadplex systems, provide their own software for the analysis of the generated data. However, the strong dependence on manual interaction make the respective solutions labour insensitive and above all not always reproducible.

Installation

# The development version from GitHub:
# install.packages("devtools")
devtools::install_github("ustervbo/beadplexr")
#
# Or with vignettes built
# devtools::install_github("ustervbo/beadplexr", build_vignettes = TRUE)

Getting started

library(beadplexr)

The package comes with a comprehensive example in the vignette 'Analysis of LEGENDplex data with beadplexr':

vignette("LEGENDplex-beadplexr")

News

beadplexr version 0.1.0

Initial release - clip the flag!

Reference manual

It appears you don't have a PDF plugin for this browser. You can click here to download the reference manual.

install.packages("beadplexr")

0.2.0 by Ulrik Stervbo, 2 months ago


https://gitlab.com/ustervbo/beadplexr


Report a bug at https://gitlab.com/ustervbo/beadplexr/issues


Browse source code at https://github.com/cran/beadplexr


Authors: Ulrik Stervbo [aut, cre]


Documentation:   PDF Manual  


MIT + file LICENSE license


Imports cluster, dplyr, drc, flowCore, fpc, ggplot2, lazyeval, magrittr, mclust, purrr, raster, tibble, tidyr, yaml

Suggests gridExtra, hexbin, igraph, knitr, rmarkdown, stringr, testthat


See at CRAN