Unified Differential Expression, Variability and Skewness Analysis for RNA-Seq Data

Provides a unified framework for the simultaneous testing of differential expression, variability, and skewness of genes using RNA-Seq data. The framework adopts a compositional data analysis approach for modelling RNA-Seq count data, applies the centered log-ratio transformation to obtain continuous variables, and uses the skew-normal distribution for statistical inference. Methods are described in Li and Khang (bioRxiv preprint, 2024, version 3) .