Automated qPCR Analysis and Visual Quality Control

Directly pipes raw quantitative PCR (qPCR) machine outputs into downstream analyses using the comparative Ct (Delta-Delta Ct) method described by Livak and Schmittgen (2001) . Streamlines the workflow from 'Excel' export to publication-ready plots. Integrates unique visual quality control by reconstructing 96-well plate heatmaps, allowing users to instantly detect pipetting errors, edge effects, and outliers. Key features include automated error propagation, laboratory master mix calculations, and generation of bar charts and volcano plots.