qPCR Ct Values to Expression Values

Computes normalized cycle threshold (Ct) values (delta Ct) from raw quantitative polymerase chain reaction (qPCR) Ct values and conducts test of significance using t.test(). Plots expression values based from log2(2^(-1*delta delta Ct)) across groups per gene of interest. Methods for calculation of delta delta Ct and relative expression (2^(-1*delta delta Ct)) values are described in: Livak & Schmittgen, (2001) .


Reference manual

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install.packages("qPCRhelper")

0.1.0 by Wilson Jr. Aala, 4 years ago


Browse source code at https://github.com/cran/qPCRhelper


Authors: Wilson Jr. Aala


Documentation:   PDF Manual  


GPL-3 license


Imports dplyr, rstatix, ggpubr, ggplot2, magrittr

Suggests knitr, rmarkdown


See at CRAN