Easy Michaelis-Menten Enzyme Kinetics

Serves as a platform for published fluorometric enzyme assay protocols. 'ezmmek' calibrates, calculates, and plots enzyme activities as they relate to the transformation of synthetic substrates. At present, 'ezmmek' implements two common protocols found in the literature, and is modular to accommodate additional protocols. Here, these protocols are referred to as the In-Sample Calibration (Hoppe, 1983; ) and In-Buffer Calibration (German et al., 2011; ). protocols. By containing multiple protocols, 'ezmmek' aims to stimulate discussion about how to best optimize fluorometric enzyme assays. A standardized approach would make studies more comparable and reproducible.


Reference manual

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0.2.4 by Christopher Cook, a year ago

Browse source code at https://github.com/cran/ezmmek

Authors: Christopher Cook [aut, cre] , Andrew Steen [aut]

Documentation:   PDF Manual  

AGPL-3 license

Imports magrittr, assertable, ggplot2, purrr, dplyr, nls2, rlang, tidyr

Suggests knitr, rmarkdown, testthat

See at CRAN