Visualising Splicing at Single-Cell Resolution

Alternative splicing produces a variety of different protein products from a given gene. 'VALERIE' enables visualisation of alternative splicing events from high-throughput single-cell RNA-sequencing experiments. 'VALERIE' computes percent spliced-in (PSI) values for user-specified genomic coordinates corresponding to alternative splicing events. PSI is the proportion of sequencing reads supporting the included exon/intron as defined by Shiozawa (2018) . PSI are inferred from sequencing reads data based on specialised infrastructures for representing and computing annotated genomic ranges by Lawrence (2013) . Computed PSI for each single cell are subsequently presented in the form of a heatmap implemented using the 'pheatmap' package by Kolde (2010) <>. Board overview of the mean PSI difference and associated p-values across different user-defined groups of single cells are presented in the form of a line graph using the 'ggplot2' package by Wickham (2007) <>.


Reference manual

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1.1.0 by Sean Wen, a year ago

Browse source code at

Authors: Sean Wen

Documentation:   PDF Manual  

GPL-3 license

Imports GenomicAlignments, GenomicRanges, IRanges, Rsamtools, plyr, ggplot2, pheatmap, ggplotify, ggpubr, scales

Suggests knitr, rmarkdown

See at CRAN