Nucleic Acid Melting Curve Analysis

Lightweight utilities for nucleic acid melting curve analysis are important in life sciences and diagnostics. This software can be used for the analysis and presentation of melting curve data from microbead-based assays (surface melting curve analysis) and reactions in solution (e.g., quantitative PCR (qPCR), real-time isothermal Amplification). Further information are described in detail in two publications in The R Journal [ <>; <>].


The MBmca package provides data sets and lightweight utilities for nucleic acid melting curve analysis and presentation on microbead surfaces.


MBmca is available on CRAN, so installation is as simple as:


You can install the latest development version of the code using the devtools R package.


install_github("MBmca", "michbur")


Reference manual

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1.0.1-3 by Stefan Roediger, 5 months ago

Browse source code at

Authors: Stefan Roediger [cre, aut] , Michal Burdukiewicz [aut]

Documentation:   PDF Manual  

GPL (>= 2) license

Imports chipPCR, stats, utils

Depends on robustbase

Suggests spelling, rmarkdown, knitr

Imported by PCRedux.

Suggested by RDML.

See at CRAN