This tool computes the probability of detection (POD) curve and the limit of detection (LOD), i.e. the number of copies of the target DNA sequence required to ensure a 95 % probability of detection (LOD95). Other quantiles of the LOD can be specified.
This is a reimplementation of the mathematical-statistical modelling of the validation of qualitative polymerase chain reaction (PCR) methods within a single laboratory as provided by the commercial tool 'PROLab' < http://quodata.de/>. The modelling itself has been described by Uhlig et al. (2015)
This is the R package 'POD'.
A tutorial is provided for the usage of the Excel macro.
To get vignettes under Linux, enter
options(browser="firefox"); browseVignettes("POD")
To get vignettes under Windows, enter
options(browser="C:/Program Files (x86)/Google/Chrome/Application/chrome.exe") browseVignettes("POD")
To use other browsers, adapt the path accordingly.