Sequence Error Filtering Pipeline for FFPE Samples

Clinical sequencing of tumor is usually performed on formalin-fixed and paraffin-embedded (FFPE) samples and have many sequencing errors. We found that the majority of these errors are detected in chimeric read caused by single-strand DNA with microhomology. Our filtering pipeline focuses on the uneven distribution of the artifacts in each read and removes such errors in FFPE samples without over-eliminating the true mutations detected in fresh frozen samples.


News

Reference manual

It appears you don't have a PDF plugin for this browser. You can click here to download the reference manual.